
pMKO.1 GFP 逆病毒载体质粒图谱序列抗性
产品名称: pMKO.1 GFP 逆病毒载体质粒图谱序列抗性
英文名称: pMKO.1 GFP 逆病毒载体质粒图谱序列抗性说明书价格报价
产品编号: Biovector105802
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
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pMKO.1 GFP
(Plasmid #10676)-
PURPOSE(Empty Backbone)
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DEPOSITING LAB
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PUBLICATION
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PRICE$65 (USD)
Add to CartAvailable to Academic and Nonprofits OnlyBACKBONE
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Vector backbonepMKO.1
- Backbone size (bp)6700
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersGFP
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primerpLXSN 5 (Common Sequencing Primers)
RESOURCE INFORMATION
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Addgene Notes
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Terms and Licenses
DEPOSITOR COMMENTS
Replaced the puromycin resistance gene from pMKO.1-puro with GFP for cell sorting. HindIII-ClaI GFP ORF from phrGFP-c (Stratagene) was cloned into pMKO.1 puro.
How to cite this plasmidThese plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMKO.1 GFP was a gift from William Hahn (Addgene plasmid # 10676)
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